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pAqpZ plasmid in E. coli
pAqpZ plasmid in E. coli
規(guī)格:
貨期:
編號(hào):B211572
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pAqpZ plasmid in E. coli
商品貨號(hào) B211572
Designations pAqpZ plasmid in E. coli
GenBank Number

U38664

Species Escherichia coli (Migula) Castellani and Chalmers
Depositors P Agre
Applications
ecause of the XhoI and SalI sites in the MCS 5' to the insert, either enzyme can be used to excise an intact open reading frame.
Vector
Construct size (kb): 3.774
Insert
DNA: cDNA
Insert lengths(kb): 0.813
Gene product: aquaporin Z [aqpZ*]
Insert Size (kb) 0.813
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/NotI--2.9, 0.84; SalI--2.9, 0.84; XhoI--2.9, 0.84.
The insert contains the following restriciton sites (approximate kb from the 5'end): SnaBI--0.25; BsmI--0.39; PacI--0.69.
The insert in this clone contains the complete open reading frame for the E. coli aquaporin Z [aqpZ*] gene plus 100 bp of the 3' untranslated region.After the 3' UTR, a SalI and XhoI restriction site have been added.Because of the XhoI and SalI sites in the MCS 5' to the insert, either enzyme can be used to excise an intact open reading frame.
Classification Enterobacteriaceae, Escherichia
References

Calamita G, et al. Molecular cloning and characterization of AqpZ, a water channel from Escherichia coli. J. Biol. Chem. 270: 29063-29066, 1995. PubMed: 7493926

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