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pTacNeo3.1 [CMCC 2301]
pTacNeo3.1 [CMCC 2301]
規(guī)格:
貨期:
編號:B211271
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pTacNeo3.1 [CMCC 2301]
商品貨號 B211271
Designations pTacNeo3.1 [CMCC 2301]
Depositors JC Hunter-Cevera
Biosafety Level 1
Vector Information
Size (kb): 5.4400000572204590
Vector: pTacNeo3.1 (plasmid)
Promoters: Promoter tac
Construction: pBR322, TAC promoter, lacZ, modified APH(3')-I
Marker(s):ampR
Construct size (kb): 5.440000057220459
Features: marker(s): ampR
promoter: tac
replicon: pMB1
Applications
expression vector
shuttle vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.5; XbaI--5.5; PstI--3.2, 2.4.
This is an expression vector capable of producing a beta-galactosidase: insert: APH(3')-I fusion protein. It can also be used as a probe.
Sequences from the TAA stop codon of APH(3')-I to the immediate downstream PvuII site encode a portion of Tn601. Sequences from the PvuII site to the PstI site are derived from Tn5.
The start codon of the modified APH(3')-I is followed by the eleventh codon (serine) of APH(3')-I. Codons 2-10 have been deleted.
The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 amino-terminal codons of beta-galactosidase, and a gene encoding a modified aminoglycoside phosphotransferase (3')-I (APH(3')-I).
The upstream translation of LacZ is out of frame with APH(3')-I.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Jennie C Hunter-Cevera, personal communication

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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