欧美精品AⅤ一区二区三区,亚洲乱码精品久久久久,99久久久国产精品免费蜜臀,精品人妻系列无码人妻免费视频,伊人久久精品无码麻豆一区,久久99人妻无码精品一区二区,国产精品免费久久久久影院仙踪林,久久99国产精品久久99果冻传媒

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > pRS318
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pRS318
pRS318
規(guī)格:
貨期:
編號:B211188
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pRS318
商品貨號 B211188
Designations pRS318
Depositors P Hieter
Biosafety Level 1
Vector Information
Size (kb): 7.4000000953674320
Vector: pRS318 (phagemid)
Promoters: Promoter T3
Construction: pRSS56 [pBluescript KS+, pBS(+)]
Marker(s):LEU2,ampR,CYH2
Construct size (kb): 7.400000095367432
Features: insert detection: lacZ'
marker(s): ampR, LEU2, CYH2
promoter: lac, T3, T7
replicon: pMB1, f1, ARSH4
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
plasmid shuffling
vector containing primer sites useful for sequencing
vector permitting RNA synthesis in vitro
vector permitting production of single-stranded DNA
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): HindIII--7.4; XbaI--7.4; PstI--7.4; BamHI--7.4.
One of a series of pBluescript-based centromere vectors (ATCC 77142-77145, 77157-77158) differing in the yeast selectable marker gene.
YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ') peptide.
Useful in plasmid shuffle experiments.
pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+).
The order of the major features in this plasmid is: LEU2 - CYH2 - f1 ori - T7 promoter - lacZ'/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

Related Products
component of:ATCC 87562
Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
福清市| 三明市| 华容县| 离岛区| 邻水| 延寿县| 永安市| 姚安县| 稻城县| 衡山县| 泽普县| 隆林| 高青县| 大理市| 乐至县| 孟连| 正定县| 德化县| 思南县| 河西区| 彩票| 清水县| 同德县| 平乡县| 沙雅县| 洛阳市| 仙居县| 梅州市| 瓮安县| 鹰潭市| 二连浩特市| 高密市| 万年县| 海城市| 周至县| 陈巴尔虎旗| 杂多县| 友谊县| 琼海市| 澎湖县| 祥云县|