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p424 MET25
p424 MET25
規(guī)格:
貨期:
編號:B211156
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p424 MET25
商品貨號 B211156
Designations p424 MET25
Depositors M Funk
Biosafety Level 1
Vector Information
Size (kb): 6.2280001640319820
Vector: p424 MET25 (plasmid)
Promoters: Promoter for expression MET25
Construction: pRS424, MET25 promoter
Marker(s):TRP1,ampR
Construct size (kb): 6.228000164031982
Features: marker(s): TRP1
marker(s): ampR
promoter for expression: MET25
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1
Applications
YX-type (expression) shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.3, 1.7, 1.0, 0.4; EcoRV--5.0, 1.4; HindIII--5.2, 1.2.
Expression from the O-acetyl homoserine sulfhydrylase (MET25) promoter is repressed when cells are grown in the presence of methionine.
High copy number shuttle expression vector.
One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mumberg D, et al. Regulatable promoters of Saccharomyces cerevisiae: comparison of transcriptional activity and their use for heterologous expression. Nucleic Acids Res. 22: 5767-5768, 1994. PubMed: 7838736

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