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BP-TIE2-W2-1
BP-TIE2-W2-1
規(guī)格:
貨期:
編號:B210934
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 BP-TIE2-W2-1
商品貨號 B210934
Designations BP-TIE2-W2-1
Species Homo sapiens, human
Depositors R Haronen
Vector
Construct size (kb): 8.899999618530273
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBacPAK8
Intact vector size: 5.538
Type of vector: phagemid
Vector end: XhoI
Vector end: EagI
Cloning sites: BstXI mLuI EcoRV HindIII DraIII ScaI
Polylinker sites: BamHI Sse8387I PstI StuI XhoI BstBI XbaI BglII Asp718I KpnI
Ecl136II SacI EcoRI XmaI SmaI EagI NotI PacI
Features (with orientation and position when available):
promoter: polyhedrin, ->
MCS: multiple cloning site, ->
stop codons: stop codons, ->
marker(s): ampR
replicon: M13
replicon: pMB1
coding sequence: AcMNPV
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: TEK
Gene name: TEK tyrosine kinase, endothelial, mutated
ORF's seq. position: DNA Seq. Acc.: L06139 ()
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 p21
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: EagI
Insert size (kb): 3.4
Cross references:
Insert lengths(kb): 3.400000095367432
Gene product: TEK tyrosine kinase, endothelial, mutated [TEK]
Insert Size (kb) 3.400
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): PstI--8.0, 0.96; BamHI--7.8, 1.1; NcoI/XhoI--5.6, 2.8, 0.5.
The insert contains the following restriction sites (approximate kb from the 5' end): PstI--0.9, 1.4; SmaI--2.9.
ATCC 87508 contains wild-type receptor tyrosine kinase TIE2 and ATCC 87507 contains a mutant gene that has a point mutation at position 2545 from C to T, resulting in an R849W substitution.
References

Vikkula M, et al. Vascular dysmorphogenesis caused by and activating mutation in the receptor tyrosine kinase TIE2. Cell 87: 1181-1190, 1996. PubMed: 8980225

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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