欧美精品AⅤ一区二区三区,亚洲乱码精品久久久久,99久久久国产精品免费蜜臀,精品人妻系列无码人妻免费视频,伊人久久精品无码麻豆一区,久久99人妻无码精品一区二区,国产精品免费久久久久影院仙踪林,久久99国产精品久久99果冻传媒

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > pBM2240
最近瀏覽歷史
更多產(chǎn)品
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pBM2240
pBM2240
規(guī)格:
貨期:
編號(hào):B210876
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pBM2240
商品貨號(hào) B210876
Designations pBM2240
Depositors M Johnston
Biosafety Level 1
Vector Information
Size (kb): 7.2500000000000000
Vector: pBM2240 (phagemid)
Construction: pRS316, lambdaMG3
Marker(s):URA3,ampR
Construct size (kb): 7.25
Features: marker(s): ampR, URA3
replicon: pMB1, CEN6/ARS4
centromere: CEN6
Applications
shuttle vector
vector for other uses
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--7.3; KpnI--5.6, 1.5; BamHI/XbaI--6.4, 0.9.
S. cerevisiae is cotransformed with the lambda clone of interest and vector linearized with an EcoRI/XhoI double digest. Ura+ colonies are tested for complementation of a mutant phenotype.
YC-type shuttle vector designed to clone yeast genes that are difficult to clone by rescuing inserts from the vector lambdaMG3 by homologous recombination.
Constructed by insertion of a 0.858 kb BamHI-XbaI fragment and a 1.5 kb KpnI fragment from lambdaMG3 into the corresponding sites of pRS316 (ATCC 77145).
The order of the major features in this plasmid is: f1 ori - lacZ - T7 promoter - KpnI/lambdaMG3/KpnI - MCS - BamHI/lambdaMG3/XbaI - T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Erickson JR, Johnston M. Direct cloning of yeast genes from an ordered set of lambda clones in Saccharomyces cerevisiae by recombination in vivo. Genetics 134: 151-157, 1993. PubMed: 8514124

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
大同县| 新乡县| 墨竹工卡县| 伽师县| 会理县| 阜康市| 辽阳县| 闽清县| 泗水县| 炎陵县| 新竹市| 乌鲁木齐市| 长武县| 育儿| 丰宁| 涟水县| 灵丘县| 大方县| 桂林市| 婺源县| 全椒县| 启东市| 石门县| 澳门| 琼结县| 高陵县| 静宁县| 虹口区| 黑水县| 绥棱县| 普格县| 武乡县| 抚宁县| 宁晋县| 万宁市| 凤庆县| 龙里县| 东山县| 山阴县| 贺州市| 沙田区|