欧美精品AⅤ一区二区三区,亚洲乱码精品久久久久,99久久久国产精品免费蜜臀,精品人妻系列无码人妻免费视频,伊人久久精品无码麻豆一区,久久99人妻无码精品一区二区,国产精品免费久久久久影院仙踪林,久久99国产精品久久99果冻传媒

熱門(mén)搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購(gòu)物車(chē) 1 種商品 - 共0元
當(dāng)前位置: 首頁(yè) > ATCC代理 > pBM2240
最近瀏覽歷史
更多產(chǎn)品
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號(hào)
  • 創(chuàng)e慧谷42號(hào)樓B幢401室
pBM2240
pBM2240
規(guī)格:
貨期:
編號(hào):B210876
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pBM2240
商品貨號(hào) B210876
Designations pBM2240
Depositors M Johnston
Biosafety Level 1
Vector Information
Size (kb): 7.2500000000000000
Vector: pBM2240 (phagemid)
Construction: pRS316, lambdaMG3
Marker(s):URA3,ampR
Construct size (kb): 7.25
Features: marker(s): ampR, URA3
replicon: pMB1, CEN6/ARS4
centromere: CEN6
Applications
shuttle vector
vector for other uses
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--7.3; KpnI--5.6, 1.5; BamHI/XbaI--6.4, 0.9.
S. cerevisiae is cotransformed with the lambda clone of interest and vector linearized with an EcoRI/XhoI double digest. Ura+ colonies are tested for complementation of a mutant phenotype.
YC-type shuttle vector designed to clone yeast genes that are difficult to clone by rescuing inserts from the vector lambdaMG3 by homologous recombination.
Constructed by insertion of a 0.858 kb BamHI-XbaI fragment and a 1.5 kb KpnI fragment from lambdaMG3 into the corresponding sites of pRS316 (ATCC 77145).
The order of the major features in this plasmid is: f1 ori - lacZ - T7 promoter - KpnI/lambdaMG3/KpnI - MCS - BamHI/lambdaMG3/XbaI - T3 promoter - pMB1 ori - ampR - CEN6/ARS4 - URA3.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Erickson JR, Johnston M. Direct cloning of yeast genes from an ordered set of lambda clones in Saccharomyces cerevisiae by recombination in vivo. Genetics 134: 151-157, 1993. PubMed: 8514124

Shipped freeze-dried
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
东阿县| 吉隆县| 合作市| 北碚区| 汝城县| 新营市| 亚东县| 正宁县| 平利县| 英吉沙县| 金山区| 天津市| 卢龙县| 南岸区| 潼南县| 曲水县| 台山市| 托里县| 西和县| 泾阳县| 永城市| 贺兰县| 科技| 奇台县| 涿鹿县| 辉南县| 永州市| 札达县| 湘潭县| 汤原县| 利津县| 静乐县| 聊城市| 日土县| 隆昌县| 马尔康县| 青海省| 奉化市| 通海县| 武清区| 墨玉县|