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hARa [CL7a-AR 160-910]
hARa [CL7a-AR 160-910]
規(guī)格:
貨期:
編號:B210810
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 hARa [CL7a-AR 160-910]
商品貨號 B210810
Designations hARa [CL7a-AR 160-910]
GenBank Number

M34233

Species Homo sapiens, human
Applications
in another host, produces protein androgen receptor (dihydrotestosterone receptor; testicular feminization)
Vector
Construct size (kb): 8.5
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pKCR-2
Intact vector size: 5.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI, EcoRI
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40
replicon: pMB1
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AR
Other gene symbols: DHTR
Genomic copy number: unique
Gene name: androgen receptor (dihydrotestosterone receptor; testicular
feminization)
Contains complete coding sequence?: N
Chromosome: X; Localization: X q11.2-q12
Tissue: testis
Type of DNA: cDNA
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 2.8
Cross references: DNA Seq. Acc.: M34233
Insert lengths(kb): 2.799999952316284
Tissue: testis
Gene product: androgen receptor (dihydrotestosterone receptor; testicular feminization) [AR]
Target Gene: androgen receptor (dihydrotestosterone receptor; testicular feminization)
Insert Size (kb) 2.800
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.0, 2.7; HindIII--8.7; KpnI--7.3, 1.35; EcoRI--4.9, 2.0, 0.64 (doublet), 0.5; PstI--4.4, 1.8, 1.7, 0.54.
Digestion with EcoRI yields two fragments of 2.0 and 0.5 kb.
The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.2, 0.4, 0.7, 0.75; EcoRI--1.9; HindIII--1.2; KpnI--1.0; NcoI--0.3, 1.75; PstI--0.5; PvuI--2.0; PvuII--0.05, 0.6; SacI--0.5, 1.2.
Encodes aa 160-910, including the DNA-binding domain and the hormone-binding domain. Also encodes 16 Gly between aa 447 and 464.
Cross hybridizes with a 6.8 kb rat liver mRNA.
pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals.
References

Govindan MV. Specific region in hormone binding domain is essential for hormone binding and trans-activation by human androgen receptor. Mol. Endocrinol. 4: 417-427, 1990. PubMed: 2342476

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