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in another host, produces protein androgen receptor (dihydrotestosterone receptor; testicular feminization)

Construct size (kb): 8.5

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pKCR-2
Intact vector size: 5.700
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI, EcoRI
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: SV40
replicon: pMB1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: AR
Other gene symbols: DHTR
Genomic copy number: unique
Gene name: androgen receptor (dihydrotestosterone receptor; testicular
feminization)
Contains complete coding sequence?: N
Chromosome: X; Localization: X q11.2-q12
Tissue: testis
Type of DNA: cDNA
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 2.8
Cross references: DNA Seq. Acc.: M34233

Insert lengths(kb): 2.799999952316284

Tissue: testis

Gene product: androgen receptor (dihydrotestosterone receptor; testicular feminization) [AR]

Target Gene: androgen receptor (dihydrotestosterone receptor; testicular feminization)

Restriction digests of the clone give the following sizes (kb): BamHI--6.0, 2.7; HindIII--8.7; KpnI--7.3, 1.35; EcoRI--4.9, 2.0, 0.64 (doublet), 0.5; PstI--4.4, 1.8, 1.7, 0.54.

Digestion with EcoRI yields two fragments of 2.0 and 0.5 kb.

The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.2, 0.4, 0.7, 0.75; EcoRI--1.9; HindIII--1.2; KpnI--1.0; NcoI--0.3, 1.75; PstI--0.5; PvuI--2.0; PvuII--0.05, 0.6; SacI--0.5, 1.2.

Encodes aa 160-910, including the DNA-binding domain and the hormone-binding domain. Also encodes 16 Gly between aa 447 and 464.

Cross hybridizes with a 6.8 kb rat liver mRNA.

pKCR-2 is an expression vector containing SV40 early and late promoters and beta-globin splicing and polyadenylation signals.