欧美精品AⅤ一区二区三区,亚洲乱码精品久久久久,99久久久国产精品免费蜜臀,精品人妻系列无码人妻免费视频,伊人久久精品无码麻豆一区,久久99人妻无码精品一区二区,国产精品免费久久久久影院仙踪林,久久99国产精品久久99果冻传媒

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Vaccinia virus ts mutant
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • [email protected]
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
Vaccinia virus ts mutant
Vaccinia virus ts mutant
規(guī)格:
貨期:
編號:B210592
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Vaccinia virus ts mutant
商品貨號 B210592
Classification Poxviridae, Orthopoxvirus
Agent Vaccinia virus ts mutant
Strain IHD-W Dts88
Applications
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D13, which encodes a 65-kDa protein thought to function as a scaffold protein for the formation of viral crescents and immature virions.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Isolation
This conditional lethal mutant, originally designated plaque isolate number 9203, was isolated from the IHD-W strain of Vaccinia by S. Dales, selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. It was assigned to Dales EM category E, inducing foci of viroplasm, aberrant membranes without spicules, and DNA paracrystals in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D13, which encodes a 65-kDa protein thought to function as a scaffold protein for the formation of viral crescents and immature virions. The non-permissive incubation temperature is 39.5oC.
Product Format frozen
Storage Conditions -70°C or colder
Comments
This conditional lethal mutant, originally designated plaque isolate number 9203, was isolated from the IHD-W strain of Vaccinia by S. Dales, selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. It was assigned to Dales EM category E, inducing foci of viroplasm, aberrant membranes without spicules, and DNA paracrystals in infected cells. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location D13, which encodes a 65-kDa protein thought to function as a scaffold protein for the formation of viral crescents and immature virions. The non-permissive incubation temperature is 39.5oC.
Vaccination is recommended for laboratory staff manipulating vaccinia, monkeypox, and cowpox viruses.
Effect on Host
CPE: enlargement, plaques with rounding and eventual sloughing
Cytopathic effects in culture include enlargement and rounding of cells, followed by death and sloughing.
Growth Medium ATCC® Medium 30-2002: Dulbecco's Modified Eagle's Medium (DMEM)
Fetal Bovine Serum
Recommended Host

Recommended Host: BSC-40 (ATCC CRL-2761)

Production host: BSC-40 (ATCC CRL-2761) Alternate hosts: MRC-5, BSC-1, HeLa, LLC-MK2
Name of Depositor S. Dales, R. Condit
References

Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807

Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570

Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
怀来县| 武功县| 喜德县| 开封市| 磴口县| 佛冈县| 稷山县| 马尔康县| 广饶县| 临武县| 政和县| 长沙市| 逊克县| 永昌县| 若羌县| 钟祥市| 黑龙江省| 红桥区| 上杭县| 天全县| 永新县| 青冈县| 郸城县| 塔河县| 隆子县| 方城县| 什邡市| 治多县| 宣化县| 乌兰察布市| 吉隆县| 浦城县| 周至县| 南溪县| 板桥市| 工布江达县| 扬州市| 禹州市| 莆田市| 洛南县| 白山市|