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Human respiratory syncytial virus
Human respiratory syncytial virus
規(guī)格:
貨期:
編號:B210557
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Human respiratory syncytial virus
商品貨號 B210557
Classification Paramyxoviridae, Pneumovirus
Agent Human respiratory syncytial virus
Strain ATCC-2012-11
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -70°C or colder
Images VR-1803 images
Comments This item was isolated in cell culture by three passages in HEp-2 cells
This strain belongs to subtype B
Effect on Host
CPE, refractile cell rounding and syncytia
Recommended Host
HEp-2 (ATCC® CCL-23™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: For best results, infection should be performed on a 70-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2), optimized for propagation (e.g. MOI 0.1-0.5 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-6 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.
Name of Depositor ATCC
Source Specimen from a clinical patient in Virginia, 2012
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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