欧美精品AⅤ一区二区三区,亚洲乱码精品久久久久,99久久久国产精品免费蜜臀,精品人妻系列无码人妻免费视频,伊人久久精品无码麻豆一区,久久99人妻无码精品一区二区,国产精品免费久久久久影院仙踪林,久久99国产精品久久99果冻传媒

Distribution host: Escherichia coli BR2943

Size (kb): 3.5000000000000000

DESCRIPTION OF VECTOR:
Intact vector size: 3.500
Type of vector: plasmid
Cloning sites:
Polylinker sites: EcoRI SalI BamHI BglII BssHII PvuI KpnI PstI PvuII ClaI NruI
SacI XhoI BglI HindIII
Construction: pVEX1191
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: P1
restriction site: PstI
marker(s): strR, spcR
restriction site: NotI
MCS: HindIII...EcoRI, ->
restriction site: NotI
other: loxP, ->
restriction site: PstI

Vector: pVEX1211 (plasmid)

Construction: pVEX1191

Marker(s):spcR,strR

Construct size (kb): 3.5

Features: marker(s): strR, spcR
other: loxP
replicon: P1
MCS: HindIII...EcoRI
restriction site: NotI
restriction site: PstI

host modification

vector for other uses

vector permitting positive selection for integration

Restriction digests of the clone give the following sizes (kb): BamHI--3.6; EcoRI--3.6; PstI--2.1, 1.1, 0.35.

To be used with pVEX2211 or pVEX2212 (ATCC 77422, 77423).

The vector is used to clone a sequence flanking the target to be deleted. The vector will be integrated by homologous recombination at the cloned sequence when spectinomycin selection is imposed in a host not expressing repA.

The deletion derivative retains one loxP site and the cmlR marker. The circularized, deleted sequence is lost from cells not expressing repA or pir.

In the presence of Cre recombinase (from E. coli EKA133 ATCC 47071), double cointegrates [pVEX1211(pVEX1212) and pVEX2211(pVEX2212)] provide two loxP sites for site-specific recombination that deletes the intervening target sequence.

Component of a vector-mediated excision system for generating in vivo deletions of large bacterial genomes, self-transmissible plasmids or temperate E. coli bacteriophages.

Differs from pVEX1212 (ATCC 77421) only in the orientation of the polylinker.

Temperature: 37.0°C