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pMK3 [WM2276]
pMK3 [WM2276]
規(guī)格:
貨期:
編號(hào):B217842
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pMK3 [WM2276]
商品貨號(hào) B217842
Designations pMK3 [WM2276]
Depositors W Messer
Applications
contains easily purifiable cassette(s) for construction lacI - lacOP cassette
Vector
Construct size (kb): 5.0
Insert
Target Gene: lacI - lacOP cassette
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): BglI--3.2, 1.45, 0.4; EcoRI--2.75, 2.15; XhoI--3.2, 1.8.
Plasmid with an XhoI promoter cassette (1.777 kb) containing the wild type lac promoter.
The vector component of this construct contains unknown sequence. This should be used only to excise the promoter-containing cassette.
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Walter Messer, personal communication

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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