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KS+SV0CAT
KS+SV0CAT
規(guī)格:
貨期:
編號(hào):B215985
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 KS+SV0CAT
商品貨號(hào) B215985
Designations KS+SV0CAT
Depositors PA Tsonis
Biosafety Level 1
Vector Information
Size (kb): 4.9000000953674320
Vector: KS+SV0CAT (plasmid)
Promoters: Promoter T3
Construction: cml from pSVOcat, pBluescript KS+
Marker(s):ampR
Construct size (kb): 4.900000095367432
Features: insert detection: CAT
marker(s): ampR
promoter: T3, T7
replicon: pMB1
Applications
expression vector
promoter-cloning vector
shuttle vector
vector permitting RNA synthesis in vitro
Comments
HindIII-ApaI fragment from pSV2cat contains internal BamHI and EcoRI sites.
Restriction digests of the clone give the following sizes (kb): SacI--4.4; Xba--4.4; BamHI--2.9, 1.6; SmaI--4.4; PstI--4.4; EcoRI--3.9, 0.32; HindIII--4.4.
Mammalian shuttle expression vector for promoter cloning with activity analysis by CAT (chloramphenicol acetyltransferase) assay. Use KS+SV2CAT (ATCC 37609) as a control.
Constructed by inserting the HindIII-ApaI fragment of pSV2cat in HindIII-ApaI digested Bluescript KS+. Contains no SV40 promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Tsonis PA, et al. CAT constructs with convenient sites for cloning and generating deletions. Nucleic Acids Res. 16: 7745, 1988. PubMed: 3166134

Panagiotis A Tsonis, personal communication

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